TaqMan-MGB探针检测人REV3L基因的毒物兴奋效应

Hormesis of human REV3L gene induced by low dose of UV detected by taqman-MGB probe

研究低剂量紫外线照射诱导人胚肺成纤维细胞(MRC5)REV3L基因表达量变化的毒物兴奋效应,用四氮唑盐比色法(MTT法)检测细胞增值抑制率,得到紫外线对MRC5毒性的剂量反应关系,并对低剂量处理组(10s和20s)和高剂量处理组(30s、60s和300s)染毒细胞,采用TaqManMGB探针,进行荧光实时定量PCR,检测REV3L基因表达量的变化.发现低剂量处理组(20s)使REV3L基因的表达量明显增高.表明低剂量紫外线照射可以引起MRC5细胞REV3L基因产生毒物兴奋效应.

The hormesis of the REV3L gene of human MRC-5 cells was induced by low doses of UV. The MTF method was used to detect the inhibition ratio of MRC-5 to obtain the dose response to UV. MRC-5 cells were treated with low doses （ 10 s, 20 s） and high doses （30 s, 60 s, 300 s） of UV and their response was measured by real time PCR using a Taqman-MGB probe. Results showed that the expression level of REV3L gene elevated following treatment by a low dose （20 s） of UV. It indicates that low doses of UV radiation cause MRC-5 cells to generate the REV3L hormesis.