荷负电气溶胶对大鼠烫伤创面愈合中细胞周期蛋白B_1、细胞周期蛋白C、增殖细胞核抗原表达的影响

Influence of aerosols on the expression of cyclin B_1, cyclin C and proliferating cell nuclear antigen in wound tissue healing of burned rat models

目的探讨荷负电气溶胶(aerosols)在烫伤创面愈合过程中对细胞周期蛋白B1(cyclinB1)、细胞周期蛋白C(cyclinC)及增殖细胞核抗原(PCNA)表达的影响。方法建立SD大鼠深Ⅱ度烫伤模型,随机分为荷负电气溶胶组和对照组,每组40只。采用免疫组织化学方法,检测创面愈合过程中cyclinB1、cyclinC及PCNA表达水平。结果荷负电气溶胶组在伤后第2天开始有cyclinC表达于基底细胞胞核,第5天胞核表达显著,第6～10天持续高水平表达。而对照组cyclinC于第3天开始表达于基底细胞胞核,第7天表达显著,第8～10天持续高水平表达。荷负电气溶胶组cyclinC表达明显高于对照组(P<0.05)。PCNA免疫组化结果显示荷负电气溶胶组和对照组在伤后第3天基底层细胞和毛囊细胞胞核均有表达,荷负电气溶胶组第5天表达显著,而对照组第7天表达显著,提示有大量细胞处于增殖状态。荷负电气溶胶组PCNA表达明显高于对照组(P<0.01)。而cyclinB1在荷负电气溶胶治疗组和对照组均于伤后第3天有部分表达于基底细胞胞浆和胞核,在第5天胞核表达明显增多,但差异无统计学意义(P>0.05)。结论荷负电气溶胶照射治疗能有效促进烫伤创面中cyclinC和PCNA的表达,促进细胞增殖,加快创面愈合。

Objective To investigate the influence of aerosols on the expression of cyclin B1, cyclin C and proliferating cell nuclear antigen （PCNA） in wound tissue healing of burned rat models. Methods Sprague Dawley （SD） rats were inflicted as the deep partial thickness burn models. Rats were randomly divided into experimental group and control group. The experimental group were treated with aerosols. Samples were collected in 1 - 10 postburn days. Immunohistochemistry and image analysis methods were conducted to examine the expression of cyclinB1, cyclin C and PCNA in both experimental and control groups. Results The expression of cyclin C in experimental group was detected in nucleus of skin basal cell on the second postburn day, increased evidently at the fifth days and sustained at high expression level up to the tenth days after treatment. The expression of cyclin C in experimental group was significantly higher than control group（P 〈 0. 05）. The expression of PCNA was first observed in skin basal cell nucleus and hair follicle cell nucleus in both experimental and control group on the third postburn day. The expression of PCNA increased evidently at the fifth days in experimental after treatment and that increased evidently at the seventh days in control group, which showed there were lots of active proliferation cell. And the difference of the expression of PCNA between experimental and control group was significant （ P 〈 0. 01 ）. The expression of cyclin B1 was detected in nucleus and cytoplasm of skin basal cell in both groups on the third postburn day, and no difference between the experimental and control group（ P 〉 0. 05）. Conclusions Aerosols can up-regulate the expression of cyclin C and PCNA in skin basal cell nucleus. Therefore the aerosols can accelerate wound tissue healing.