木兰假丝酵母不对称还原制备(S)-CHBE

Production of(S)-CHBE by asymmetric reduction with whole cells of Candida magnoliae as catalyst

利用木兰假丝酵母催化的4-氯乙酰乙酸乙酯(COBE)立体选择性还原反应,制备了具有光学活性的手性化合物(S)-4-氯-3-羟基丁酸乙酯((S)-CHBE).分别考察培养条件、共底物种类及浓度、转化时间、转化温度、底物添加量、细胞浓度和pH值等因素对产物得率和对映体过量值(e.e.)的影响.结果表明,在30℃、pH 6.0的磷酸钾缓冲液(100 mmol/L)中添加10 g/L共底物葡萄糖振荡转化时,9 g/L好氧培养的木兰假丝酵母能将12.1 g/L的4-氯乙酰乙酸乙酯几乎全部地转化为(S)-4-氯-3-羟基丁酸乙酯,且e.e.值达到85.2%.

Optically active ethyl （S）-4-chloro-3-hydrobutanoate（（S）-CHBE）,a useful chiral synthon,was prepared by stereoselective bioreduction of ethyl 4-chloro-3-oxobutanoate （COBE） with Candida magnoliae cells. Effects of culture condition, co-substrates and their concentrations, reaction time, temperature, pH, substrate concentration, and cell concentration on the yield and enantiomeric excess （e. e. ） of product were examined in detail. The results showed that 99.2% of 12.1 g/L COBE was reduced to （S）-CHBE with an e. e. value of 85.2% by 9 g/L cells of Candida magnoliae at 30℃ in phosphate buffer of pH 6.0 added with 10 g/L of glucose.