人正常子宫颈移行上皮重症联合免疫缺陷小鼠模型的建立

Establishment of animal model of human cervical tissue in SCID mice

目的探讨人正常子宫颈移行上皮重症联合免疫缺陷小鼠(SCID鼠)模型建立的可行性及其生物学特性。方法获取正常子宫颈组织的移行带标本,接种于12只SCID鼠皮下,随机分成3组,分别在2、4、6周取出其皮下移植物行HE染色、免疫组化及人乳头瘤病毒(HPV)DNA的检测。结果所有小鼠的移植物均存活,病理证实存在宫颈鳞柱状上皮并具有基底膜结构,上皮细胞免疫组织化学染色ki-67阳性,HPV DNA检测为阴性。结论初步建立了人正常子宫颈移行上皮SCID鼠模型,为进一步研究子宫颈上皮HPV感染致癌变过程提供了理想的动物模型。

Objective To establish a mouse model system of human cervical epithelium and investigate its biological fetures. Methods Normal cervical tissue specimens were obtained from the transformation zone of hysterectomy specimens. tissue were placed subdennally into 15 severe combined immunodeficient （SCID） mice. The mice were randomly divided into three groups. The implant was saved for pathology, immunohistochemistry and HPV DNA examination at 2nd,4th, 6thweeks. Results All mice had survived implant with identifiable stratified squamous epithelium and the basement membrane. Proliferation epithelium was demonstrated by expression of the Ki67. HPV DNA in tissue was hegative. Conclusion The establishment of the animal model of human cervical epithelium is successful, which can provide an ideal model for the investigation of natural history of cervical epithelium HPV infectious cavcinogenesis.