呼宁胶囊的毒性实验研究

Research on the Toxicity of Huling Capsule

目的观察和测定呼宁胶囊对小白鼠的LD50和对大白鼠的长期毒性。方法急性毒性实验给小白鼠一次灌胃给药后,测定其半数致死量及其95%的可信限。长期毒性实验给3个剂量组和1个空白对照组的大白鼠连续灌胃给药90天,观察大鼠出现的毒性反应,剂量毒性效应的关系,主要靶器官毒性反应的性质和程度等。结果呼宁胶囊对小白鼠的LD50为3981mg.Kg-1,其95%的可信限为4685.5～3382.5mg.Kg-1。连续用药90天,大鼠血液分析、肝肾功能化验结果未见显著差异,重要脏器做病理解剖学及病理组织学检查均未见病理学改变。

Objective： Observing and determing the LD80 of HuLing capsules for mice and the long-term toxicity for rats. Methods： Acute toxicity test： To determine the LD50 and 95 % reliability limit after giving mice the medicine once by po. Long-term toxicity test： continuously giving rats of three groups of different dosage of the rugs and one blank control group for 90 days, observing the toxicity reaction of the rats, the dose-effect relationship, the nature and degree of the toxicity reaction of the main target organs. Results： The LD50 from HuLing capsules for mice is 3981mg·Kg^-1, the 95% reliability limit is 4685.5-3382.5 mg·Kg^-1. Incessantly giving 90 days of medicine, no distinct differences found in the hemometry and the liver and kidney function tests of the rats, no pathological changes found in the anatomicopathological and histopathological tests of their important organs.