波兰小麦(Triticum polonicum L.)高分子麦谷蛋白亚基多样性分析

Allelic Variations of High Molecular Weight Glutenin Subunits in Triticum polonicum L.

利用SDS-PAGE电泳技术鉴定和分析了72份不同来源的波兰小麦高分子量谷蛋白亚基组成。结果表明,供试波兰小麦材料中有10种亚基组合方式。其中,亚基组合类型(N,20)出现的频率最高,高达41·67%;其次是(Null,7)亚基组合。在各位点的等位变异中,Glu-A1位点出现3种等位变异,以Null出现的频率最高(84·7%);而在Glu-B1位点上检测到了5种等位变异。不同地理来源的波兰小麦高分子量谷蛋白亚基组合类型从1到10种不等,尤其是来源于欧洲地区的波兰小麦,具有检测到的所有亚基组合类型,存在较高多样性。

Allelic variation of high molecular weight glutenin subunits （HMW-GS） among 72 accessions of Triticurn polonicum L. were analyzed by using one dimensional SDS - PAGE. A total of 10 subunit combinations were observed. Among which, the highest frequency of subunit combinations （N, 20） was 41.67%, following the second frequent was （N, 7） subunit combination. Three and 5 allelic variations were found in Glu-A1 and Glu -B1 locus, respectively. The most abundant subunit combinations were detected in those materials from Europe.