摘要
建立了一种土壤中尿素的高效液相色谱分析方法.以纯水为流动相,采用ODS色谱柱,流量1mL/min,测定波长190nm.测定尿素的线性范围为2～20μg/mL,检测限为0.3μg/mL;回收率为96.3～104.5%,相对标准偏差为3.8～5.2%.本法可用于各类土壤中尿素的测定.
A high performance liquid chromatography （HPLC） was proposed for urea in soil. In this method, a ODS column was used, pure water was used as a mobile phase. The flow rate was 1.0mL/ min, the detect wavelength was 190nm. The linear range was 2-20/lg/mL,with a 0.0-200mg/L detection limit of 0.3μg/mL. The recovery rate was 96.3-104.5%, the relative standard deviation was 3.8 -5.2%. This method could be applied to the determination of content of urea in all kinds of soils.
出处
《安徽技术师范学院学报》
2005年第5期1-3,共3页
Journal of Anhui Agrotechnical Teachers College
基金
安徽省高校科研基金项目(2005KJ153)
关键词
高效液相色谱法
土壤
尿素
High performance liquid chromatography
Soil
Urea
