长裙竹荪子实体酸提水溶性多糖DiA的分离、纯化及组成单糖的鉴定

Isolation,Purification of Soluble Polysaccharide DiA by Acid Extraction from Dictyophora indusiata and Identification of ITs Monosaccharide Components

长裙竹荪(Dictyophora indusiata)子实体的3%三氯乙酸提取液经乙醇沉淀,去蛋白后得到多糖粗制品。粗制品再经二次DEAE-纤维素柱层析分离和Sephadex G-200柱纯化得到竹荪水溶性多糖DiA。经Sepharose 4B柱层析、聚丙烯酰胺凝胶电泳和醋酸纤维薄膜电泳鉴定,DiA为—单一物质。纸层析和气相层析表明,DiA的单糖组成为葡萄糖和甘露糖,其摩尔比为:0.29:1.00。多糖的分子量约为168000。

A soluble polysaccharide DiA was obtained through being extracted from dried fruitbodies of Dictyophora indusiata by 3% trichloreacetic acid, precipitated by cthanol, deproteinized by protease method and Sevag method, then isolated twice by DEAE-cellulose column chromatography and further puried by Sephadcx G-200 column. DiA was proved homogeneous by chromatography on Sepharose 4B column, acetate cellulose membrane electrophoresis and polyacrylamide gel electrophoresis. Identified by paper chromatography and gas chromatography, it was proved that the components of DiA were composed of D-Glc and D-Man with a molar ratio of 0.29:1.00 and that its molecular weight was estimated to be 168000.