糖尿病模型小鼠的肠道菌群与血糖关系的探讨

STUDY ON THE RELATIONSHIP BETWEEN THE BLOOD SUGAR LEVELS AND MICROFLORA IN DIABETIC MOUSE MODEL.

目的:通过降糖物质使糖尿病模型小鼠血糖降低,探讨血糖变化与肠道菌群的关系。方法:采用腹腔注射链脲菌素(STZ)的方法建立小鼠糖尿病模型;在小鼠糖尿病造模前后及降血糖后,采用活菌计数方法,分别检测小鼠肠道主要的菌群,并采用血糖仪测定小鼠血糖值,利用统计学方法研究二者的关系。结果:造模成功小鼠血糖升高(>11.1mmol/L,P<0.05)时,肠道益生菌(双歧杆菌,乳酸杆菌)、梭菌的数量降低(P<0.05);经降糖物质处理后,小鼠血糖降低(P<0.05),肠道益生菌、梭菌的数量恢复到正常值(P<0.05)。但是,某些条件致病菌,如大肠杆菌、肠球菌、真菌等,在整个实验里呈递增状态(P<0.05)。结论:不同降糖物质对肠道菌群影响相似;小鼠肠道益生菌的变化趋势与血糖变化呈负相关,肠杆菌、肠球菌、真菌与血糖变化的规律不明显。

Objective： To discuss the relationship between the blood sugar levels and microflora, changing in the whole experiment process, by establishing the diabetic mouse model and treating with two types of substances that reduce the blood sugar levels. Methods： The diabetic mouse model was established by injecting Streptozotocin （STZ） in abdominal cavity. The numerical value of blood sugar and microflora was measured during the experiments, and then the relativity was discussed by the means of statistics method. Results： When blood sugar levels were beyond the nomal range （ 〉 11.1 mmol/L , P 〈 0.05）, probiotics （Bifidohacteriurn, Lactohacillus） and Clostridium significantly decreased （P 〈 0.05） . Through oral administration of the hypoglycemic substances, the blood sugar levels , probiotics and Clostridium resumed the normal rang （P〈0.05） . It was noteworthy that Enterobacter, Enterococcus and Fungus were always at the high level （P 〈0.05） . Conclusions： The various hypoglycemic substances are able to develop the same change on microflora. The change of the blood sugar levels are contrary to probiotics. Microflora may play an important role during occurring and/or develop in Diabetes mellitus.