摘要
采用硫酸铵盐析、超滤、ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０柱层析，从绿豆芽中提纯了超氧物歧化酶。酶的纯化程度达到每ｍｇ蛋白含３１９４．７Ｕ，总酶活回收率为３８．９，纯化倍数为３０，７．酶理化性质研究表明，所得酶在２７０ｎｍ紫外光区有一吸收峰；聚丙烯酰胺凝胶电泳显示２条谱带；氨基酸分析表明含３０８个氨基酸残基；在６０℃处理４０ｍｉｎ，酶活损失很小；在ｐＨ２～１１范围活性稳定；ＫＣＮ、ＥＤＴＡ对酶活性具抑制作用。
Sod from green bean sprouts was purified through(NH4)2SO4.solt-surplussion,ultrafiltration and DEAE-sephadex A 50.The puration exhibited 3 1 94.7(L/protein activity),thetotal recovery rate of enzyme activity was 38.9 and the purification times was 30.7.The studyon its physicochemical properties showed that the ready-owned enzyme had a absorption peakat 270 nm in ultraviolet spectrum and two derivative spectra were observed through polyacry-lamide get electrophoresis.The analysis of.amino acid showed that it had 308 amino acidresidues and the enzyme activity loss was little when treated at 60 celsius for 40 minutes.Between pH2 and pH11,it had a stable activity but could be inhibited by KCN,EDTA.
出处
《江西农业大学学报》
CAS
CSCD
1996年第2期206-212,共7页
Acta Agriculturae Universitatis Jiangxiensis
关键词
超氧物歧化酶
绿豆芽
纯化
超滤
superoxide dismutate
green bean sprouts
purification
ultrafiltration
