西洋参皂苷对高脂肪食小鼠脂肪和胰脂肪酶活性的影响

Effect of Saponins of Panax quinquefolium on Inhibiting High-Fat Diet Induced Obesity and Lipase Activity of Mice

通过体外、体内试验研究了西洋参总皂苷、单体皂苷对胰脂肪酶活性及小鼠体重和子宫周围脂肪组织重的影响。体外试验结果表明:西洋参茎叶总皂苷为0.5 g/L时,对胰脂肪酶活性的抑制率为90%;人参皂苷Rc,Rb1,Rb2对胰脂肪酶活性均显示很强的抑制作用,当质量浓度为0.5 g/L时抑制率分别为100%、96%、97%;体内试验结果表明:西洋参茎叶皂苷对体重影响变化不大,但可以明显降低子宫周围脂肪质量。这说明西洋参茎叶总皂苷和人参皂苷Rc,Rb1,Rb2可以抑制胰脂肪酶活性。因此西洋参茎叶总皂苷的抗肥胖作用可能是由于西洋参总皂苷中的人参皂苷Rc,Rb1,Rb2抑制小肠吸收食物脂肪的结果。

Quinquefolium on pancreatic lipase activity was performed by measuring the release rate of oleic acid from triolein. For in vivo experiments, female ICR mice were fed a high fat diet with or without 1% or 3 % saponins from stem and leaf of Panax quinquefolium for 8 weeks. 1% or 3 % crude saponins from stem and leaf Panax quinquefolium inhibited pancreatic lipase activity. The results showed that at the concentration of 0.5 g/L, saponins from stem and leaf Panax quinquefolium inhibited pancreatic lipase activity by 90%. At the concentration of 0.5 g/L, ginsenosides Rc, Rb1 and Rb2 inhibited pancreatic lipase activity by 100%,96% and 97% respectively. 1% or 3% crude saponins from stem and leaf Panax quinquefoliwn suppressed the increases in parametrial adipose tissue weight, when mice were fed a high-fat diet. It is demonstrated that the anti-obesity effects of saponins from stem and leaf Panax quinquefolium in high-fat diet treated mice may be due to the inhibition of intestinal absorption of dietary fat by ginsenosides Rc, Rb1 and Rb2.