以桑色素为模板的分子烙印聚合物的制备、结合特性及其在薄层色谱固定相方面的应用

Preparation and Binding Characteristic of a Morin-imprinted Polymer and Its Application to Thin Layer Chromatography Stationary Phase

以桑色素为模板,选用不同的功能单体和致孔剂制备了一系列分子烙印聚合物,采用结合实验考察了它们对底物的吸附特性,发现以丙烯酰胺为功能单体,以乙酸乙酯为致孔剂制得的烙印聚合物对底物有很好的选择性,同时对一些结构相似的化合物具有一定的结合力.Scatchard方程研究结果表明,在研究的浓度范围内聚合物形成了一类等价的结合位点,其平衡离解常数为0.877 mmol/L,对模板分子的最大表观结合量为59.18μmol/g.可认为桑色素分子中3,7,4′位的羟基与功能单体的氢键作用是分子烙印聚合物具有底物选择性的主要因素.以该分子烙印聚合物为薄层色谱固定相,可以把模板分子从结构相似的化合物中分离出来,最小分离度为1.19.

A series of Morin-imprinted polymers were prepared and their adsorption properties for the template were evaluated by binding experiments. The results show that the polymer prepared by using acrylamide as the functional monomer, CH3CO2Et as the porogen exhibited a higher selectivity for Morin and good affinity to its structural analogues. The molar ratio of template to functional monomer in the pre-polymeric mixture was optimized. Scatchard analysis shows that a equal class of binding sites were formed in the imprinted polymer in the studied concentration range. The dissociation constant and the apparent maximum number of the binding sites were 0.877 mmol/L and 59.18 μmol/g, respectively. The function of the functional groups of the template in the formation of complementary interacting site in the polymer was discussed. It was concluded that the hydrogen bonds formed between the C3-OH, C7-OH, C4＇-OH of Morin and acrylamide played an important role in molecular recognition. Using the Morin-imprinted polymers as thin layer chromatographic stationary phase can separate the template from the other compounds. The minimal value of Rs to its structural analogue compound was 1.19.