苯并(a)芘对鲫鱼肝脏EROD活性的影响

Effects of benzo(a)pyrene exposure on 7-ethoxyre sorufin O-deethylase activity in Carassius auratus liver.

研究了典型多环芳烃类有机污染物苯并(a)芘(BaP)对鲫鱼(Carassius auratus)肝脏7-乙氧基-3-异吩唑酮-脱乙基酶(EROD)活性的影响。结果表明,注射后96 h,10和100 mg.kg-1处理组肝脏EROD活性被明显诱导,分别为对照组的2.3(P<0.05)和3.1倍(P<0.01)。肝脏EROD活性随着时间延长继续升高,至注射后14 d,1 mg.kg-1处理组鲫鱼肝脏EROD活性为对照的3.0倍(P<0.001),而100 mg.kg-1处理组则高达5.8倍(P<0.001)。鲫鱼肝脏EROD活性可作为反映BaP暴露水平的生物标志物。BaP对鲫鱼的最低效应浓度为1 mg.kg-1(鱼体重)。

This paper studied the effects of a typical organic pollutant benzo（a）pyrene （BaP） on the activity of 7- ethoxyre sorufin O-deethylase（EROD）in Carassius auratus liver. The results showed that BaP could induce EROD activity significantly. After injected with 10 and 100 mg·kg^- 1 BaP for 96 h, the EROD activity was as 2.3 （ P 〈 0.05） and 3.1 （P 〈 0.01） times as that of the control, and at the 14th day after injected with 1 and 100 mg·kg^- 1 BaP, the activity was up to 3.0 （P 〈 0. 001） and 5.8 （P 〈 0. 001） times of the control, respectively. The EROD activity in C. auratus liver could reflect the level of BaP exposure, and might be a promising biornarker of the organic pollutant. The minimum effect concentration of BaP on C. auratus was 1 mg·kg^ - 1 （BW）.