摘要
通过在培养基中加入一定浓度的Cd2+,从土壤中分离筛选出2株具有较强镉抗性及产酸能力的细菌JL-4和JC-9.经初步鉴定,JL-4和JC-9分别属于芽孢杆菌属(Bacillus sp.)和黄单胞菌属(Xanthomonas sp.).将菌株JL-4和JC-9接种到添加不溶性镉(100mg·L-1)的培养液中28℃培养48 h,接菌处理比不接菌对照培养液中有效镉含量分别增加1 392.3%和1 410.8%,培养液pH分别降低了2.87和3.18.将JL-4和JC-9分别接入含不同浓度重金属镉(100、200mg·kg-1)的土壤中28℃培养20 d,接菌处理比不接菌对照土壤中有效镉含量分别增加17.02%~100%(JL 4)和36.17%~61.80%(JC-9).菌株JL-4和JC-9的最适生长温度均为28℃,最适pH值分别为7和8.除对镉具有抗性外,两菌株对重金属铅、铜、镍、锌也具有一定抗性.
In this paper, cadmium-resistant bacteria were isolated from heavy metals-contaminated soils through agar plate containing Cd^2+ as 3CdSO4·8H2O. The strains JL-4 and JC-9 were identified as Bacillus sp, and Xanthomonas sp., respectively, based on their morphological, physical, and biochemical characteristics, In the liquid medium containing Cd^2+ (100 mg· L^-1) and inoculated with JL-4 or J C-9, the available Cd^2+ content in supernatant was increased by 1 392.3% (JL-4) or 1 410.8% (JC-9), compared with the control, and the pH was decreased to 2.87 (JL-4) or 3.18 (JC-9), Soil cadmium (100,200 mg·kg^-1) activation experiments showed that the available Cd^2+ content in soil inoculated with JL-4 or JC-9 was increased by 17.02 % - 100 % (JL-4) or 36.17 % - 61.80 % (JC-9), compared with the control. The optimum temperature for JL-4 and JC- 9 was 28℃, and the optimum pH for them was 7 and 8, respectively. The two strains could also be resistant to Pb^2+(800mg·L^-1),Cu^2+ (50mg·L^-1),Ni^2+(50mg·L^-1),and Zn^2+(200mg·L^-1).
出处
《生态学杂志》
CAS
CSCD
北大核心
2005年第11期1357-1360,共4页
Chinese Journal of Ecology
基金
国家自然科学基金项目资助(40371070)
关键词
重金属污染
镉
菌株筛选
活化
heavy metals contamination, cadmium, strain screening, activation