摘要
目的:观察胆结石患者胆汁对人胆管癌细胞HCCC-9810生长的影响,探讨胆结石与胆管癌发生、发展的关系。方法:应用四甲偶氮唑蓝比色法检测18份胆结石患者胆汁和8份正常胆汁对HCCC-9810增殖的影响,应用流式细胞仪测定细胞周期和凋亡。结果:胆结石患者胆汁与正常胆汁比较,明显促进HCCC-9810细胞增殖,用胆结石患者胆汁处理48 h的HCCC-9810细胞增殖指数显著上升(P<0.01),胆结石胆汁组(47%±10%)S期细胞比例比正常胆汁组(23%±3%)明显增高(P<0.01),G0/G1期细胞比例(42%±8%)比正常胆汁组(63%±10%)明显降低(P<0.05)。结论:胆结石患者胆汁具有潜在的促增殖活性,胆结石与胆管癌的发生、发展关系密切。
Objective: To explore the effects of bile from patients with biliary calculus on the growth of human cholangiocarcinoma cells HCCC -9810 and the potential correlation between biliary calculus and cholangiocarcinoma. Methods:Biliary calculus bile ( CB ) and normal bile (NB) specimens were used for this study. The proliferative effects of bile were measured by methabenzthiazuron (MTY) assay and cell cycle and apoptosis were analyzed by flow cytometer. Results: CB can significantly promote the proliferation of HCCC -9810 cells, HCCC-9810 proliferative index increased significantly after treated with 1% CB for 48 h (P 〈0.05 ). The S phase fraction of CB (47% ± 10% ) increased remarkably ( P 〈0.05) compared with that of NB (23% ± 3% ), and the CB percentage of G0/G1 phase (42% ± 8%) decreased remarkably (P〈0.05) compared with (63% ± 10% ). Conclusions: CB can promote the proliferation of human cholangiocarcinoma HCCC -9810 cells.
出处
《中国民康医学》
2005年第10期561-562,共2页
Medical Journal of Chinese People’s Health
关键词
胆结石
胆汁
增殖
biliary calculus
bile
cell proliferation