靶标抗性点突变基因型检测技术

Genotyping Techniques of Identifying Point Mutation Associated with Target Resistance

杀虫剂的不合理使用导致害虫的抗药性日趋严重。抗药性是一种涉及一个或几个昆虫基因的遗传特征。靶标抗性通常与点突变相关,对抗性相关的点突变的快速检测诊断是抗性治理的基础之一。评述了5种基因型检测技术———单链构象多态性、固相微型测序、双向等位特异PCR扩增、实时荧光定量PCR和焦磷酸测序的优缺点及其在突变检测中的应用。

Overapplication of insecticides made the insect pests serious resistance. Insecticide resistance is an inherited characteristic involving in one or more insect genes. Target resistance is usually associated with point mutation （s）. Identifying the point mutation （s） is very important in resistance management. Comments on five genotyping techniques and their application in the identification of point mutation, including single-stranded conformational polymorphism, solid-phase minisequencing, bi-directional PCR amplification of specific alleles, real-time fluorescence quantitative polymerase chain reaction and pyrosequencing, were presented.