苯酚硫酸法测定栀子水溶性多糖含量

Determination of water soluble polysaccharide in fructus gardeniae

目的建立中药栀子中水溶性多糖含量的测定方法。方法用苯酚-浓硫酸显色法,以葡萄糖作为标准品,于490 nm处测吸光度。结果栀子中多糖含量为:4.96%,RSD=1.43%。结论该方法简便易行,结果稳定可靠,适合用于中药栀子中水溶性多糖含量的测定。

Aim To establish a simple and accurate method for the determination of polysaccharide in Fructus Gardeniae. Method The UV speetrophotometry was used to determine polysaeeharide in Fruetus Gardeniae, max =490 nm. Results Polysaeeharide in Fruetus Gardeniae was 4.96% , RSD = 1.43% , Conclusion The method was rapid,simple,reliable and accurate.