摘要
目的研究CD44v6与E-钙黏附素(E-cad)在早期宫颈鳞癌中的表达,探讨其在淋巴结转移中的作用及临床意义。方法采用反转录聚合酶链反应(RT-PCR)与免疫组织化学法对21例正常宫颈上皮、63例无淋巴结转移宫颈鳞癌、23例有淋巴结转移宫颈鳞癌标本中CD44v6、E-cad的mRNA和蛋白表达进行检测。结果宫颈鳞癌组织中CD44v6mRNA的表达高,而在正常宫颈几乎不表达;E-cad的mRNA在正常宫颈组织表达高而在宫颈鳞癌表达低,有淋巴结转移和无淋巴结转移的宫颈鳞癌组织差异均有统计学意义(P<0.05)。CD44v6蛋白在宫颈鳞癌表达增高而E-cad蛋白表达降低,且在有转移和无转移的宫颈鳞癌组织中差异有统计学意义(P<0.05),且CD44v6和E-cad的表达与淋巴结转移相关(P<0.05)。结论CD44v6、E-cad通过改变早期宫颈鳞癌细胞的黏附力而分别发挥了促进和抑制宫颈鳞癌淋巴结转移的作用,可作为评估预后的生物学指标。
Objective To study the expression and clinical significance of CD44v6 and E-cadherin（E-cad） in squamous cervical carcinoma. Methods Twenty-one cases of normal cervical tissue, 63 cases of squamous cervical carcinoma without lymph node metastasis,23 cases of squamous cervical carcinoma with lymph node metastasis were detected for the mRNA and protein expression of CD44v6 and E-cad by means of RT-PCR and immunohistochemical method. Results CD44v6 was negatively expressed in normal cervical epithelium,but the expression of CD44v6 went up in squamous cervical carcinoma,transepithelial carcinoma of cervix uteri-invasive carcinoma of cervix uteri. CD44v6 had positive correlation with clinical staging and histological grading of the carcinoma.The positive rate of CD44v6 in people with lymph node metastasis was higher than that in those without lymph node metastasis. Conclusion The positive expression of CD44v6 has close relationship with lymph node metastasis. Expression of CD44v6 is important for evaluating the prognosis of cervical cancer.
出处
《中国药物与临床》
CAS
2005年第10期747-750,共4页
Chinese Remedies & Clinics
基金
太原市科委攻关基金资助项目(并科学2003064)
