摘要
目的探讨IL-12联合IL-2在抗P.berghei红内期感染中的免疫调节作用。方法BALB/c小鼠接种5×105个感染P.berghei的RBC,同时分别给予0.03μg/dIL-12、40U/dIL-2或IL-12联合IL-2处理,连续用药6d,观察小鼠原虫血症变化及存活时间。通过淋巴细胞增殖转化试验、T淋巴细胞亚群测定及NK细胞杀伤活性检测等方法,观察细胞免疫应答水平。结果IL-12联合IL-2处理组可较单独IL-12或单独IL-2及感染对照组显著推迟原虫血症达峰值的时间与明显延长小鼠的存活时间,IL-12或联合IL-2组小鼠在原虫血症达30%时开始死亡,而单独IL-2或感染对照组小鼠在原虫血症低于13%时已全部死亡。IL-12联合IL-2处理组脾淋巴细胞总数、CD4+与CD8+百分比、3H-TdR掺入量及NK细胞杀伤活性均较其它各处理组及感染对照组显著升高。结论IL-12与IL-2二者可协同促进脾淋巴细胞的增殖及增强NK细胞杀伤活性以诱导抗P.berghei红内期感染的免疫保护作用。
Objective To study the combined effects of IL-12 and IL-2 on the development of protective immunity to blood-stage P.berghei. Methods P.berghei infected BALB/e mice were treated with 0.03μg of IL-12 alone or in combination with 40 U of IL-2 once a day for six consecutive days. Parasitemia was monitored and survival time was calculated. Cellular immune responses were elvaulated by lymphocyte proliferation test, CD4^＋ and CD8^＋ T cells counts and NK cell eytotoxieity. Results Combined treatment with IL-12 and IL-2 could significantly delayed the peak of parasitemia and prolonged survival time of the P.berghei infected mice when compared with the treatment with IL-12 or IL-2 alone. Moreover, the survivalship of the infected mice treated with IL-12 alone or with a eomination of IL-12 and IL-2 started to drop at 30% parasitemia, whereas mice treated with IL-2 alone or without treatment all died before reached 13% of parasitemia. Further, mice received the combine treatment with IL-12 and IL-2 showed significant increases in the numbers of splenic lymphoeytes, the percentages of CD4^＋ and CD8^＋ T cells, the levels of 3H-TdR incorporation of splenic cells and the activities of NK cells. Conclusion The results indicated that combine treatment with IL-12 and IL-2 could exert synergetie effect on the development of protective immunity to blood-stage P.berghei, probably through the enhancement of lymphocyte proliferation and NK cell eytotoxieity.
出处
《热带医学杂志》
CAS
2005年第6期734-737,755,共5页
Journal of Tropical Medicine
基金
江苏省自然科学基金(No.BK97157)。
