蛋白芯片、抗体检测及RT-PCR技术研究不同HCV感染人群的病原学差异

Study on the Different HCV Infection Groups by Protein Chips, ELISA and RT-PCR

目的采用多种不同的检测方法探讨静脉吸毒人群与非静脉吸毒人群中丙型肝炎病原学的差异。方法89份静脉吸毒人员血清及40份非静脉吸毒HCV感染者血清分别应用分片段蛋白质芯片、酶联免疫吸附试验(ELISA)及荧光定量逆转录聚合酶链反应(RT-PCR)进行检测丙型肝炎病毒(HCV)抗体及RNA,比较两种不同人群中HCV感染的病原学差异及三种检测方法在以上两种样本中检测结果的差异。结果在静脉吸毒人群中HCV感染率为88.76%(79/89),感染者血清HCVRNA载量较非吸毒HCV感染者血清HCVRNA载量高,前者为1×103.10拷贝/毫升,后者为1×102.26拷贝/毫升(t=1.986,P=0.049)。应用HCV分片段蛋白芯片技术检测的敏感性为98.23%。结论静脉吸毒人群中HCV感染率较高,感染者中血清HCVRNA载量较非静脉吸毒者高。研究显示,蛋白芯片技术是一种高效的检测技术,具有临床应用价值。

Objective To investige the difference of HCV infection between the injection drugs users with HCV infection and the non-injection drugs users with HCV infection by protein chips, ELISA and RT-PCR. Method The sera of 89 injection drugs users and 40 non-injection drugs users were assayed by reverse transcriptasepolymerase chain reaction（RT-PCR） using established primers for HCV, enzyme linked immuosorbent assay（ELISA） for HCV-Ab and protein chip for peptide antibody for HCV, Results HCV infection was detected in 88.76%（79/89）, the sera download of HCV-RNA in the injection drugs users with HCV is higher than that in the non injection drugs users with HCV（t=l.986,P=O.049）, it is 1×10^3.10 copys/ml and 1×10^2.36 copy/ml, respectively. There were 112 sera detected posive by protein chip for peptide antibody for HCV in 114 sera that was detected positive by ELISA. Conclusion The infection ratio is high in the injection drugs users and injection drug is one of the most risk factor of the HCV infection. The sera download of HCV-RNA in the injection drugs users with HCV is higher than that in the non injection drugs users with HCV. The protein chip for peptide antibody for HCV was an efficient technique and maybe used clinically.