广东黑石顶厚壳桂(Cryptocarya chinensis)不同年龄级遗传多样性

Genetic Diversity of Cryptocarya chinensis Life Stages in Heishiding, Guangdong Province

采用ISSR分子标记对广东省黑石顶地区厚壳桂(Cryptocaryachinensis)不同年龄级(成体、小树、幼苗)的遗传多样性进行研究。结果表明,和具有相同生活史特征的物种相比,黑石顶厚壳桂遗传多样性水平较低,总的基因多样性为0.0901;不同年龄级遗传多样性由成体-小树-幼苗逐渐降低,分别为0.0880、0.0793、0.0648。3个年龄级间的遗传分化为Gst=0.1410,st=0.0659(AMOVA)。这一地区厚壳桂种群低的遗传多样性是由于这一地区森林片断化后,种群隔离导致不同地区种群间基因流的不畅通、个体数量缩小使得种群内遗传漂移作用加剧。但种群世代间遗传分化可能由取样误差导致的遗传漂变所产生。

ISSR markers were used to examine genetic diversity in three different life stages of Cryptocarya Chinensis in Heishiding, Guangdong Province. The results indicated the level of genetic diversity of C. chinensis （H T= 0.0901） was low compared with those species with the similar life history. The genetic diversity decreased from adults, juveniles to seedlings, and the degree of genetic differentiation between life stages was Gst = 0. 1410, Фst = 0. 0659. Low gene flow between populations may cause the low genetic diversity in C. chinensis. However, the genetic differentiation might be attributed to sampling deviation.