猪多杀性巴氏杆菌(PM)PCR诊断技术的建立

Establishment of PCR for diagnosing pasteurella multocida

参照文献报道的猪多杀性巴氏杆菌(PM)基因组的部分序列,设计合成引物,建立了PM的PCR诊断技术,并对各反应条件进行优化。本实验建立的PM的PCR扩增片段大小为457 bp。以建立的PCR方法对临床送检的可疑病猪的肺部病料进行了特异性检测和敏感性试验及生化鉴定。结果表明PCR检测阳性结果与猪多杀性巴氏杆菌的形态染色、培养特性和生化鉴定结果一致,该PCR方法在临床诊断中具有应用价值。

A pair primers were designed based on the sequence of PM published in Genbank, the size of the amplified frafement is 457 bp. By optimizing the PCR system and procedure, a PCR-based assay for detecting Pasteurella multoceda （PM） was developed. The experiment showed that the result of PCR detection was correspondent with bacterium isolation.