乙型肝炎患者血清前S1抗原与HBV的血清标志物的关系

Study on Correlation of Serum Pre-S1 Protein and HBV Serum Marker from Patients Infected with HBV

目的分析前S1抗原与其他HBV血清标志物的关系,探讨其临床意义。方法收集HBsAg(+)血清标本214例,HBsAg(-)血清标本159例,采用ELISA和PCR方法对其标本373例进行血清前S1抗原、HBV-DNA和HBV血清标志物的检测,分析前S1抗原与HBeAg等血清标志物的关系。结果不同HBV血清标志物的标本前S1抗原和HBV-DNA的阳性率无显著差异(P>0.05)。在98例前S1抗原阳性标本中有91例HBV-DNA检测为阳性,而前S1抗原检测阴性的275例中,有209例HBV-DNA呈阴性。两种方法的符合率达79.6%。经卡方检验两种方法检测的结果存在显著相关性(P<0.01)。对前S1抗原和HBeAg检测结果的分析,HBeAg(+)标本中前S1抗原阳性率为85.2%,而HBeAg(-)标本中,前S1抗原阳性率仅为32.5%,前S1抗原与HBeAg也具有相关性。结论前S1抗原能够敏感地反映HBV复制情况,与HBeAg和HBV-DNA有很好的相关性。

Objective To observe changes of serum Pre S1 protein in different kinds of HBV marker combination model in patients infected with HBV and to explore its clinical significance. Methods To collect HBsAg（ ＋ ） positive serum（214）, HBsAg（-） negative serum（159）, using ELISA and PCR method, blood samples of 373 of serum Pre S1 protein, HBV-DNA and in different kinds of HBV marker combination model were analyzed about relationship of Pre S1 and HBeAg. Results The rates of positive of Pre S1 protein and HBV-DNA marker serum samples in different kinds of HBV these is no significant difference separately （P 〉 0.05）. The HBV-DNA （91）in Pre S1 protein positive blood sampies （98） were positive, but the HBV- DNA （209） in Pre S 1 protein negative blood samples （275） were negative, the coincided rates of two examine methods were 79.6%. X2 test, the results of two examine methods there is significant difference separately （ P 〈 0.01）. The analyzed of Pre S1 protein and HBeAg for results, the positive rotes of Pre S1 protein in HBeAg（ ＋ ）blood samples were 85.2%, but the positive rates of Pre S1 protein in HBeAg（-） blood samples were 32.5 %, the Pre S1 and HBeAg there is significant difference separately. Conclusion Pre S1 can reflect the replication of HBV sensitively, Pre S1 and HBeAg HBV-DNA both there is significant relativity.