一种检测低内毒素浓度的传感器

Development of a Piezoelectric Sensor for Detection of Low Endotoxin Concentration

内毒素(ET)是造成内毒素血症及并发症的因子,准确检测其浓度对于合理诊治该类疾病具有重要意义。利用石英晶体的液体阻尼效应,开发了一种用于检测水中内毒素含量的压电生物传感器。实验结果表明,传感器的最大频移值与内毒素浓度(0.1pg/ml^10ng/ml)的对数值以及频移曲线中d(Δf)/dtmax出现时刻与其浓度(0.01pg/ml^10ng/ml)对数值都存在很好的线性关系。后者较前者具有测量时间缩短和最低检测限下降的优点。与传统的检测方法相比具有简单、定量性好、自动化程度高和最低检测限低的优点。

Endotoxin is an important factor which can lead to endotoxemia and complication. Accurate detection of its concentration is very useful for the diagnosis and treatment of these diseases. A piezoelectric biosensor for detecting endotoxin was developed, which was based on liquid damping effect of quartz crystal resonator. The test results showed that the maximal frequency shift of sensor is linearly dependent on the logarithm value of concentration of endotoxin （0.1 pg/ml-10 ng/ml）. The time which d（Af）/dtmax appeared in frequency shift curve was also linearly dependent on the logarithm value of concentration of endotoxin （0. 01 pg/ml -10 ng/ml）. The detection time was shortened and the minimal limit of detection was decreased using the second method, thus the proposed sensor is much simpler, more precise and has more lower limit of detectiing detection of endotoxin when compared with the conventional methods.