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Cox-2抑制剂联合放疗对小鼠Lewis肺癌作用的实验研究 被引量：1

Effects of Cox-2 inhibitor combined with radiotherapy in mice bearing Lewis tumors
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摘要 目的了解选择性环氧化酶-2(Cox-2)抑制剂罗非昔布(Rofecoxib)联合放疗对负载Lewis肺癌荷瘤小鼠的抗肿瘤效应.方法建立负载Lewis肺癌肿瘤细胞的C57BL/6小鼠的动物模型,肿瘤接种后12天直径增至0.5～0.7 cm时应用医用直线加速器6MV-X线对荷瘤小鼠行局部肿瘤分次放疗,5 Gy/次,第12、14、16、18天;将浓度为4%的Rofecoxib灌胃200 μl/只,1次/日,第12～16天.游标卡尺测量肿瘤大小;电子天平称肿瘤湿重;免疫组化法测肿瘤组织微血管密度;HE染色测肺转移灶数目;肺称重;观察小鼠的生存期.结果治疗后15天,放疗联合Cox-2抑制剂组小鼠肿瘤体积分别比生理盐水组、单纯放疗组和Cox-2抑制剂组减小了4倍、1倍和2倍;肺重量分别减轻了约1.6倍、1倍及1倍;肺转移灶数减少了约2.7倍、1.4倍及1.3倍.联合治疗组小鼠生存期明显延长.免疫组化法显示联合治疗组小鼠的肿瘤组织中微血管密度明显减少.结论对荷瘤小鼠,放疗联合Cox-2抑制剂治疗能够抑制负载LLC肿瘤细胞的小鼠肿瘤生长和转移,延长小鼠生存期,起到协同增效作用.两者的联合应用可能是一种独特的抗肿瘤治疗途径.
作者 任志刚 李平 谢正强
机构地区 四川大学华西医院肿瘤放疗科 郧阳医学院附属东风医院肿瘤科
出处 《实用肿瘤杂志》 CAS 2005年第5期404-408,共5页 Journal of Practical Oncology
关键词 肺肿瘤 前列腺素内过氧化物合酶 小鼠 肿瘤细胞 培养的 放射疗法 免疫组织化学
分类号 R734.2 [医药卫生—肿瘤] R73-36 [医药卫生—肿瘤]
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参考文献11

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同被引文献8

  • 1马绍英,王仲文,王小莉,邢小红.环氧化酶-2抑制剂对肺癌细胞的增殖抑制和放射增敏的实验研究[J].肿瘤,2005,25(2):140-143. 被引量：7
  • 2Xu XC. COX-2 inhibitors in cancer treatment and prevention, a recent development. Anticancer Drugs, 2002, 13 : 127-137.
  • 3Kumumbail PG, Stevens AM, Gierse JK, et al. Structural basis for selective inhibition of cyclooxygenase-2 by anti-inflammatory agent. Nature, 1996,384:644-648.
  • 4Kishi K, Petersen S, Petersen C, et al. Preferential enhancement of tumor radioresponse by a cyclooxygenase-2 inhibitor. Cancer Res, 2000,60:1326-1331.
  • 5Raju U, Nataka E, Yang P, et al. In vitro enhancement of tumor cell radiosensitivity by a selective inhibitor of cyclooxygenase-2 enzyme: mechanistc considerations. Int J Radiat Oncol Biol Phys, 2002,54:886-894.
  • 6Yip-Schneider MT, Sweeney CL, Jung SH, et al. Cell cycle effects of nonsteroidal anti-inflammatory drugs and enhanced growth inhibition in combination with gemcitabine in pancreatic carcinoma cells. J Pharmacol Exp Ther,2001,298 :976-985.
  • 7Lira JW, Kim H, Kim KH. Expression of Ku70 and Ku80 mediated by NF-kappa B and cyclooxygenase-2 is related to proliferation of human gastric cancer cells. J Biol Chem,2002 ,277 :46093-46100.
  • 8王凤玮,梁克殷,蔚伯,沈瑜,盛修贵.放射线诱导人鼻咽癌细胞系凋亡的研究[J].中华放射肿瘤学杂志,1998,7(1):9-12. 被引量：8

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实用肿瘤杂志
2005年 第5期

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