狼疮肾炎患者尿单核细胞趋化蛋白-1检测的临床意义

Clinical significance of urine MCP-1 determination in patients with lupus nephritis

目的探讨狼疮肾炎患者尿单核细胞趋化蛋白-1(MCP-1)水平变化在狼疮肾炎中的临床意义。方法采用酶联免疫吸附试验(ELISA)方法对23例狼疮肾炎,13例原发性肾炎和12名健康对照者的尿MCP-1水平进行检测,比较分析其与肾脏损害及其与肾脏组织病理活动性指数(AI)的相关关系。结果狼疮肾炎患者尿MCP-1水平为(1130±403)pg/mg肌酐,显著高于对照者(141±109)pg/mg肌酐(P<0.01)和原发性肾炎患者(452±174)pg/mg肌酐(P<0.01)。狼疮肾炎患者尿MCP-1水平与24h尿蛋白(r=0.558,P<0.05)、血肌酐(r=0.869,P<0.01)、尿N-乙酰-D-氨基葡萄糖酶(NAG)(r=0.617,P<0.05)及肾脏组织病理AI(r=0.751,P<0.01)呈显著正相关。结论狼疮肾炎患者尿MCP-1水平可作为了解肾脏活动性病变的一个分子指标。

Objective To explore the clinical significance of urine monocyte chemoattractant protein- 1 （MCP-1） in patients with lupus nephritis （LN）. Methods Urine MCP-1 was detected by Enzyme-linked immunosorbent assay （ELISA）. Correlation analysis of urine MCP-1 and renal injury, urine MCP-1 and the activity indices of renal histopathologic change were evaluated. Results Urine MCP-1 level in LN patients （1130± 403） pg/mg Cr was significantly higher than that of normal controls （141±109） pg/（mgCr） （P〈0.01） and primary nephritis patients （452±174） pg/mg Cr （P〈0.01）. Urine MCP-I was significantly correlated with proteinuria （r= 0.558, P〈0.05）, serum creatinine level （r=0.869, P〈0.01）, urine NAG （r=0.617, P〈0.05） and the activity indices （AI） of renal histopathologic change （r=0.751, P〈0.01）. Conclusion Urine MCP-1 can be used as a molecular maker to evaluate disease activity of patients with lupus nephritis.