不同取代羧甲基壳聚糖体外酶降解的研究

Study on the Biodegradation of Carboxymethyl Chitosan with Different Substitution in vitro

目的:研究不同取代羧甲基壳聚糖(CMC)的生物降解性。方法:通过体外酶解试验,用粘度测定法研究了在溶菌酶作用下CMC的分子量。结果:溶茵酶作为催化CMC降解的非专一性酶,能有效地催化CMC降解;酶解反应程度与酶浓度成线性关系;随着羧甲基壳聚糖取代度的增加,CMC的降解速度加快。结论:通过控制取代度,可以得到不同生物降解要求的羧甲基壳聚糖。

Objective： To study biodegradation of series of carboxymethyl chitosan with different substitution. Method ： Though enzymatic hydrolysis in vitro, the molecular weight of CMC was measured by the method of viscosity measurement under the effect of lysozyme. Result： The lysozyme could be used as the non-specific enzyme to catalyze the degradation of CMC effectively. There was a linear relationship between the degree of enzymatic hydrolysis reaction and enzyme concentration. The velocity of biodegradation of CMC grew rapidly while the substitution degree of carboxymethyl chitosan increased. Conclusion： By controlling the degree of substitution, various kinds of carboxymethyl chitosan meeting different biodegrading requests were obtained.