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雷公藤内脂醇对EAE大鼠MCP-1mRNA表达的影响 被引量：4

The Effect of Triptolide on MCP-1 mRNA Expression in Wistar Rats with Experimental Autoimmune Encephalomyelitis
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摘要 目的探讨雷公藤内脂醇(TRD)对EAE大鼠MCP-1mRNA表达的影响。方法将Wistar大鼠免疫后7d随机分成4组:EAE组、TRD1组、TRD2组、TRD3组,给予不同剂量的药物注射,取脑和脊髓制成石蜡切片,进行HE和MCP-1mRNA的原位杂交染色,比较各项临床指标。结果TRD在每只鼠每日40g剂量下影响EAE大鼠MCP-1mRNA的表达。结论雷公藤内脂醇可增加循环中的糖皮质激素从而抑制EAE中MCP-1mRNA的表达,抑制EAE。 Objective To study the immunosuppressive effects of triptolide to MCP-1 mRNA expression of EAE Wistar rots. Methods GPSCH, CFA and PT-immtmized Wistar rats are divided randomly into four groups, group EAE, group TRD 1, group TRD 2 and group TRD 3. The brains and spinal cords were removed for HE staining and in situ hybridizations （ISH） staining. Results Group TRD 3 （40μg TRD · rat^-1 · d^-1） implies a therapeutic effect. Conclusion TRD arrests the development of EAE in rats, partly through increasing circulating glucocorticoid and down-regulating the expression of MCP-1 mRNA.
作者 刘颖 刘华 马存根 马太花 梁丽云 米亚英
机构地区 山西医科大学第一临床医学院神经科 山西大同大学脑科学研究所 山西大同大学脑科学研究所
出处 《深圳中西医结合杂志》 2005年第5期278-280,283,共4页 Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
基金 山西省2003年度回国留学人员科研基金资助项目(2003-09)
关键词 雷公藤内脂醇(TRD) EAE MCP-1 MRNA TRD EAE MCP-1
分类号 R-332 [医药卫生]
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参考文献12

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深圳中西医结合杂志
2005年 第5期

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