高温高湿环境下颅脑火器伤后兔脑HSP70变化

Changes of heat-shock protein 70 in the brain of rabbits with craniocerebral missile injury in hot and humid environment

目的研究高温高湿环境下颅脑火器伤后兔脑热休克蛋白70(HSP70)的变化及其作用.方法采用高温高湿颅脑枪伤模型,24只新西兰大白兔随机分成常温(22.0±0.5℃、相对湿度50%)对照组、常温(22.0±0.5℃、相对温度50%)枪伤组和高温高湿(39.0±0.5℃、相对温度80%～85%)枪伤组,每组8只.采用Western blot法检测脑组织、淋巴细胞中HSP70,应用化学发光和X线片显示,凝胶图像分析仪定量分析.结果颅脑枪伤受高温高湿环境作用后,脑皮质、下丘脑和淋巴细胞的HSP70显著增高;淋巴细胞中的HSP70与大脑皮质、下丘脑的变化趋势相一致.结论脑组织和淋巴细胞中HSP70在高温高湿环境颅脑火器伤时显著增加.

Objective To study the changes of heat shock protein 70 （HSP70） in the brain of rabbits with craniocerebral missile injury （CMI） and their impact on the injury in a hot and humid environment （HHE）. Methods Twenty-four New Zealand white rabbits were randomized into 3 equal groups, namely normal temperature （NT） control group （subjected to treatment at temperature of 22.0±0.5 ℃ with relative humidity of 50%）, NT gunshot group with CMI and HHE gunshot group with CMI. The rabbits in the latter two groups were subjected to normal temperature of 22.0±0.5 ℃ with relative humidity of 50% and environmental temperature of 39.0±0.5 ℃ with relative humidity of 80% to 85%, respectively, after establishment of CMI models. HSP70 in the brain tissues and lymphocytes was detected by Western blotting and visualized using chemo- luminescence and X-ray films, followed by quantitative gel image analysis. Results Expressions of HSP70 in the cortex, hypothalamus and lymphocytes increased apparently in HHE gunshot group, and the changes of HSP70 in the lymphocytes were consistent with those in the cerebral cortex and hypothalamus. Conclusion HSP70 expression in the brain tissues and the lymphocytes increase evidently after MCI in HHE.