大肠癌脾虚证bcl-2基因表达与健脾康复汤的调节作用

Expression of bcl-2 gene in spleen deficiency syndrome in colorectal carcinoma and the regulatory effect of Jianpikangfu decoction

目的探讨大肠癌脾虚证与bcl-2基因表达的相关性及中药健脾康复汤的调节作用。方法选择中晚期大肠癌脾虚证患者45例,随机分为治疗组和对照组。治疗组口服健脾康复汤,每日1剂,同时给予西医对症支持治疗;对照组仅给予西医对症支持治疗。疗程为2个月。治疗前后分别检测唾液淀粉酶活性及组织标本bcl-2基因表达。结果大肠癌脾虚证患者酸刺激后的唾液淀粉酶活性显著下降,组织标本bcl-2基因表达阳性率明显升高。治疗组酸刺激前后唾液淀粉酶活性差值与对照组比较有显著差异(t=7.822,P<0.01);治疗组组织标本bcl-2基因表达阳性率治疗后较治疗前显著降低(χ2=4.286,P<0.05),对照组bcl-2基因表达阳性率治疗前后无显著差异。结论中药健脾康复汤对大肠癌脾虚证患者酸刺激后唾液淀粉酶活性下降的趋势有抑制作用,对bcl-2基因表达有调节作用。

Objective To investigate the correlation between spleen deficiency syndrome in colorectal carcinoma and bcl-2 gene expression, and observe the regulatory effect of Jianpikangfu decoction. Methods Forty-five advanced colorectal carcinoma patients with spleen deficiency were randomized into Jianpikangfu decoction treatment group with also symptomatic treatment with western medicine and control group in which the patierts were given expactant treatment with western medicine. The activity of salivary amylase and bcl-2 expression in the tumor tissues were detected before and after the treatment. Results Jianpikangfu decoction in combination with western medicine treatment produced more obvious inhibition of reduction in salivary amylase activity than exclusive western medicine treatment （t=7.822, P〈0.01）, and significantly lowered the positivity rate of bcl-2 expression （x^2=-4.286, P〈0.05） in the tumor tissues, which, however, displayed no obvious changes in response to exclusive western medicine treatment. salivary amylase activity and regulate bcl-2 gene expression in Conclusion Jianpikangfu decoction can inhibit the decrease in colorectal carcinoma patients with spleen deficiency syndrome.