桔林油脂酵母的生长和淀粉酶产生

Growth and Amylase Production of Lipomyces kononenkoae

在模拟淀粉废水的连续培养中对桔林油脂酵母及突变体的生长和淀粉酶产生进行了研究。在低稀释率（０．０３～０．２５ｈ－１）、ｐＨ４．８、３２℃条件下出现最大淀粉酶产量。Ｄ＝０．１０ｈ－１时，突变体所产生的淀粉酶活性为２３ｕ／ｍｌ，最大淀粉酶产量为１．４５ｕ／ｍｌ·ｈ。细胞生物量的产量在Ｄ＝０．１５ｈ－１时最大，细胞生物量的产率取决于葡萄糖的消耗，其产率值为０．７９。培养液中的淀粉浓度平均减少８９％。

Growth and amylase production of L.kononenkoae mutant was studied in continuous culture on simulated starchy wastewater.Under the condition of D=0.10h-,PH4.8,32℃,the highest amylase activity was 23u/ml.The maximum amylase production of the mutant was 1.45u/ml·h.Cell mass productivity reached a maximum at D=0.15h1.Yield of cell massbased on reducing sugars consumed at D=0.15h- was 0.79.During the chemostat experiments starch concentrations were reduced by a value of 79%.