蒿甲醚和青蒿琥酯对感染曼氏血吸虫小鼠治疗作用的初步研究

Therapeutic effect of artemether and artesunate in mice infected with Schistosoma mansoni

目的了解蒿甲醚和青蒿琥酯对小鼠曼氏血吸虫作用的效果。方法将小鼠随机分成12个实验组及1个对照组,以皮下注射的方法,每鼠接种约80条尾蚴,接种尾蚴46d后,分别以蒿甲醚或青蒿琥酯灌胃治疗,第1天,分别以400、300、200mg/kg的剂量1次灌胃;第2～7天,则每天分别按以上剂量的半量灌胃,7d灌胃的总剂量分别为1600、1200、800mg/kg。总量1剂组,在第7天,分别按1600、1200、800mg/kg剂量1次灌胃。另设感染阳性对照组,不加治疗。结果蒿甲醚7日疗法1600、1200、800mg/kg剂量组减虫率分别为53.0%、49.0%和53.0%,减雌率为78.0%～82.0%,总量1剂组效果与7日疗法基本相同。青蒿琥酯7日疗法相应剂量减虫率分别为16.0%、37.0%和49.0%。结论蒿甲醚和青蒿琥酯对小鼠曼氏血吸虫具有一定的杀虫效果,蒿甲醚在疗效和毒性方面稍佳。

Objective To explore the therapeutic effect of artemether and artesunate on adult Schistosoma mansoni in experimental mice. Methods The mice were administered intragastrically with artemether or artesunate 46 days after being infected with cercariae of S. mansoni subcutaneously. On Day 1,a dose of 400, 300, 200 mg/kg of artemether or artesunate was administered to the mice. From Day 2 to Day 7, a half above dose was administered. On Day 7, the single-dose groups were administered with artemether or artesunate at the dose of 1 600, 1 200, 800 mg/kg, meanwhile, an infected group of mice was served as control, untreated. Results With 7-day therapy of artemether at the dosage of 1 600, 1 200, 800 mg/kg, the worm reduction rates were 53%, 49% and 53%, respectively, and female worm reduction rates were from 78%--82%, compared with the control group. The therapeutic effects of artemether on single-dose groups were similar. The worm reduction rates, with 7-day therapy of artemether at the dosage of 1 600, 1 200, 800 mg/kg , were 16%,37% and 49%, respectively, compared with the control group. Conclusion The efficacy of therapy with artemether and artesunate on S. mansoni infection mice were relatively well. Concerning the therapeutic effect and toxicity, artemether is slightly better than artesunate.