大鼠局灶性脑缺血再灌注后纹状体内肿瘤坏死因子-α及白细胞介素-1β的动态变化被引量：2

The changes of striatum TNF-α and IL-1β in rats after focal cerebral ischemia-reperfusion

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摘要目的观测大鼠局灶性脑缺血再灌注早期不同时间缺血侧纹状体内肿瘤坏死因子-α(TNF-α)及白细胞介素-1β(IL-1β)的动态变化。方法对60只雄性Sprague-Dawley(SD)大鼠采用大脑中动脉线栓法建立局灶性脑缺血模型,并随机将大鼠分为对照组、缺血再灌注组,每组30只。缺血再灌注组在大鼠脑缺血1 h后进行再灌注。在再灌注1、3、6、122、4 h,应用ELISA方法检测缺血侧纹状体TNF-α及IL-1β的动态变化。结果与对照组相比,脑缺血大鼠再灌注1 h缺血侧纹状体TNF-α含量开始明显增高[(9.91±1.57)μg.(g Pro)-1],而IL-1β在再灌注3 h后才有明显升高[(8.75±1.59)μg.(g Pro)-1],TNF-α及IL-1β均于再灌注6 h升高最为明显[(12.62±1.64,12.24±1.23)μg.(g Pro)-1],然后逐渐下降,且至再灌注24 h二者仍维持在较高水平[(9.81±1.53,8.52±1.49)μg.(g Pro)-1](均P<0.05)。结论局灶性脑缺血再灌注后早期TNF-α及IL-1β的含量增高可能在缺血再灌注性脑损伤的病理过程中起重要作用。 Objective To observe the changes of tumor necrosis factor-alpha（TNF-α ） and interleukin-1 beta （IL-1β） in ischemic striatum after focal cerebral ischemia-reperfusion. Methods Sixty male Sprague-Dawley rats were randomly divided into 2 equal groups： control group undergoing sham operation; ischemia-reperfusion group undergoing thread embolism of the left middle cerebral artery occlusion to cause focal ischemia for 1 hour and then undergoing reperfusion. Then the rats in the 2 groups were re-divided into 10 subgroups. Rats in each subgroup were decapitated at 1, 3, 6, 12 and 24 hours after reperfusion respectively. ELISA method was used to detect the levels of TNF-α and IL-1β in the ischemic striatum. Results Compared with control group, the levels of TNF-α increased significantly after 1 hour reperfusion [ （9.91 ± 1.57） μg· （g Pro）^-1 ], while the up-regulation of IL-1β was detected significantly after 3 hours reperfusion[ （8.75 ± 1.59） μg· （g Pro）^-1 ]. Both of them elevated to peak level after 6 hours reperfusion[ （ 12.62 ± 1.64, 12.24 ± 1.23） μg· （g Pro）^-1 ]. Then they decreased gradually, and remained in high level after 24 hours reperfusion[ （9.81 ± 1.53, 8.52 ± 1.49）μg· （g Pro）^-1 ] （ all P 〈 0.05）. Conclusions The up-regulation of TNF-α and IL-1β may play an important role in cerebral ischemia-reperfusion injury.

作者周红乔健

机构地区东南大学附属中大医院神经内科复旦大学上海医学院神经病学研究所

出处《现代医学》 2005年第5期294-297,共4页 Modern Medical Journal

关键词脑缺血再灌注肿瘤坏死因子-Α 白细胞介素-1Β cerebral ischemia reperfusion tumor necrosis factor-α interleukin-1β

分类号 R743.33 [医药卫生—神经病学与精神病学]

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