单发佐剂性关节炎大鼠脊髓单胺类递质及代谢产物含量分析

Changes of Spinal Monoamine Transmitter and Their Metabolites in Rats with Adjuvant Monoarthritis

目的探讨慢性关节炎症痛时脊髓单胺类递质和代谢产物含量及释放量的变化.方法采用完全弗氏佐剂在大鼠一侧后肢造成单发性关节炎模型,用高效液相色谱法分别测定致炎前及致炎后1～9周大鼠脊髓灌流液和脊髓背角单胺类递质及其代谢产物的含量.结果 (1)致炎5周后脊髓去甲肾上腺素释放量增加而同期含量降低,炎症1～9周期间脊髓3-甲氧基-4-羟基苯乙二醇释放量明显减少;(2) 5-羟吲哚乙酸脊髓释放量在第5周降低而同期含量升高;(3) 3,4-羟基苯乙酸脊髓释放量明显降低但含量变化不明显,高香草酸未见有明显改变.结论单胺类递质及代谢产物参与了慢性炎症痛期间内源性镇痛系统的作用.

Objective To explore law of monoamine transmitters and their metabolites in the model rats of adjuvant- induced monoarthritis. Methods Monoarthritic rat model was produced by injecting water- in - oil complete Freun＇d adjuvant （CFA） into the right tibio - tarsal joint, and then monoamine transmitters and their metabolites in the spinal cord before and during 1 - 9 weeks of CFA injection were measured by reversed - phase high performance liquid chromatography. Results （ 1 ） The release of NE from spinal perfusate were increased in arthritic rats 5 weeks after inoculation, and the content of NE in dorsal spinal cord were decreased at the same period, while MHPG showed a significant reduction from 1 - 9 weeks. （2） The spontaneous release of 5 - HIAA was decreased at 5 weeks after injection of CFA. At the same time, the content of 5 - HIAA in dorsal spinal cord showed a opposite changes. （3） During the period of inflammatory pain, the release of DOPAC decreased markedly, but its content had a little change. The release of HVA had not any change. Conclusion Spinal monoamine transmitter and their metabolites take part in the action of endogenous anfinociception in adjuvant - induced monoarthritic inflammatory oain.