参附注射液预防心动过缓的实验观察

Effects of Shenfu Injection Against Bradycardia

目的探讨参附注射液预防缓慢性心律失常的作用。方法将48只大鼠平均分为两个实验部分,每部分随机分为3组(每组8只)。第一部分:三组大鼠经尾静脉分别注射0.9%NS 5mL/kg(第一组)、参附注射液5mL/kg(第二组)、参附注射液10mL/kg(第三组),每日2次,连续5d;用乙酰胆碱(ACh)制造大鼠心动过缓的模型,用BL-420生物记录仪记录不同处理大鼠注射Ach前后心率和P-R间期的变化。第二部分:三组大鼠除不给Ach外,其他处理和观察时间同第一部分;采血用硫代巴比妥酸法测定丙二醛的含量,用硝酸还原酶法以测定一氧化氮含量,用黄嘌呤氧化酶法测定超氧化物歧化酶含量。结果与第一组比较,注射Ach后第二和第三组大鼠心率明显高于第一组(P<0.01);P-R间期三组大鼠注射Ach后均高于注射前(P<0.05或P<0.01);与第一组比较,注射Ach后第二和第三组大鼠P-R间期较第一组明显缩短(P<0.05或P<0.01)。此外,和第一组相比,第二组、第三组大鼠血清MDA、NO含量下降,而血清SOD含量增加,差异均有统计学意义(P<0.01)。结论参附注射液有一定的抗心动过缓的作用,其作用机制可能与血清SOD的含量增加及NO、MDA的含量减少有关。

Objective To investigate the effects of shenfu injection on bradycardia. Methods Fourty eight rats were averagely grouped into two parts. In each part, rats were randomly divided into three groups. Group 1, 0.9%NS（Smg/kg） was injected group 2 and group 3. Shenfu Injection （5mg/kg and 10mg/kg） was injected into tail vein two times for 5 days. Part one： Bradycardic models were set up with acetylcholine in rats. Electrocardiogram was recorded by BL - 420. Part two： Except for Ach injection, the three subgroups were treated as sameas the part one did. Maleic Dialdehyde （MDA）, Nitric Oxide （NO）, Superoxide Dismutase （SOD） contents of serum were measured respectively by Thibabituric Acid, Nitrate Reductase, Xanthine Oxidase. Results The heart rate was enhanced rapidly and P- R phase was shortened in group 2 and 3 than those in group 1 after Ach injection （P 〈0.05 or P 〈0.01）. The P-R Phase was longer after Ach injection than before in three groups. In addition, the MDA, NO contents of serum were decreased in group 2 and3（ P 〈0.05）. But the SOD contents of serum were increased in group 2 and 3 （ P 〈 0.01 ）. Conclusion Shenfu injection has antagonistic effects agaist bradycardia. The effects may be due to the decrease of MDA and NO and the increase of SOD.