RUNX3基因364位点C→T突变与胃癌关系的研究

Relationship between RUNX3 gene 364 locus C→T mutation and gastric cancer in Chinese

目的:研究RUNX3基因C364T突变在我国胃癌高、低发区普通人群和胃癌患者中的分布,H．pylori 感染者胃粘膜的RUNX3基因C364T突变率,探讨此突变与我国胃癌发生的关系。方法:采用PCR-限制性片段长度 多态性(RFLP)分析法检测胃癌高发区169名普通人、86例胃癌患者和胃癌低发区。192名普通人和92例胃癌患者的 RUNX3基因多态性。同时比较胃癌低发区普通人胃粘膜H．pylori阳性和阴性者的RUNX3突变率。结果:在胃癌 高、低发区,胃癌患者RUNX3基因C364T突变频率与普通人群无显著差异(X2=0．57和0．16,P>0．05)。与肿瘤类 型也无明显关系。低发区H．pylori阳性者粘膜中,RUNX3基因突变率也无显著增高。结论:RUNX3基因C364T突变 可能不是我国胃癌高、低发区胃癌的遗传易感因素。而且H．pylori感染导致胃癌形成可能不由RUNX3基因C364T 突变参与。

AIM： To study the frequency difference of RUNX3 gene 364 locus C→T mutation between normal people （controls） and gastric cancer （GC） patients, and mutation in gastric mucosa of subjects with H. pylori infection. METHODS： Genomic DNA was extracted from peripheral blood and gastric mucosal biopsy specimens of normal people and GC patients in lower or higher prevalence region. Gene mutation was analyzed by PCR-RFLP. RESULTS： The frequency of RUNX3 T/T genotype was no significant difference between controls and GC in lower （X^2 = 0.57, P 〉 0.05） or higher prevalence region （X^2 = 0.16, P 〉 0.05）. A higher mutation rate in mucosal tissue infected with H. pylori was not discovered. CONCLUSION： RUNX3 gene C364T mutation may be not a genetic susceptibility to GC in Chinese. The mutation is impossibly involved in the pathway of H. pylori infection resulting in gastric carcinoma.