糖尿病大鼠骨质疏松与过氧亚硝基阴离子关系的免疫组化研究

Immunohistochemistry study on the role of peroxynitrite in diabetic rat osteoporosis

目的:探讨过氧亚硝基阴离子(ONOO-)与糖尿病(DM)骨质疏松(OP)的关系。方法:用链脲佐菌素 (STZ)诱导糖尿病大鼠模型12周后,进行骨密度(BMD)检查、观察股骨胫骨形态学改变,应用免疫组化法检测骨中 和高糖溶液培养成骨细胞(OB)中的诱导型一氧化氮合酶(iNOS)、ONOO-水平的变化。结果:高糖溶液培养OB中的 iNOS和ONOO-升高;DM大鼠骨中的iNOS和ONOO-升高、BMD降低、骨形态学呈退行性改变。结论:骨中iNOS、 ONOO-增加可能是糖尿病OP的发病机制之一。

AIM： To explore the role of peroxynitrite （ONOO^-） in pathogenesis of diabetic osteoporesis （OP）. METHODS： The diabetic rat model was induced by streptozotocin （STZ） for 12 weeks. Bone mineral density （BMD） was measured. HE staining was employed to observe the morphologic changes in bone tissues. The method was used to detect the content of iNOS and ONOO- in the bone tissues and osteoblasts （OB） cultured at high concentration of glucose. RESULTS： The results showed that high concentration of glucose increased the contents of iNOS and ONOO^- in OB. The BMD was decreased and the bone tissues were deteriorated, while iNOS and ONOO^- increased in the bone tissues of diabetic rats, which were reversed by aminoguanidine. CONCLUSION： High content of iNOS and ONOO^- in bone is probably one factor in diabetic osteoporesis （OP）.