过氧亚硝基阴离子在肝纤维化形成中的作用

Effect of peroxynitrite anion (ONOO- ) on the development of hepatic fibrosis

目的:探讨过氧亚硝基阴离子(ONOO-)在肝纤维化形成过程中所起的作用。方法:采用复合因素 建立肝纤维化(HF)动物模型的同时,用一氧化氮合酶抑制剂L-NNA(20mg·kg-1·d-1)给大鼠灌胃(HF+NNA),于实 验4周末测定血浆脂多糖(LPS)、NO2-／NO3-含量;采用免疫组化的方法检测肝组织硝基酪氨酸(NT)的表达;用VG 染色观察肝组织纤维化程度。结果:在HF+NNA组血浆NO水平明显低于HF组(P<0．01),HF组NT表达最强,纤 维化程度最严重。结论:ONOO-能促进肝纤维化的发展。

AIM： To explore the effect of peroxynitrite anion （ONOO^-） on development of hepatic fibrosis. METHODS： The hepatic fibrosis （HF） of Wistar rats was established by giving complex pathogens. The rats were intra-gastrically infused with 0.9% salt （1.5 mL/d） and inhibitor of nitric oxide synthase （NOS）, L- NNA （20 mg·kg^-1 ·d^-1）, respectively. At the end of the 4th week, the levels of plasma LPS and NO2^-/NO^3- were detected. The expression of nitrotyrosine （NT） in hepatic tissue was examined by immunohistochemical techniques. The degree of hepatic fibrosis was observed by VG staining. RESULTS： The level of plasma NO in HF ＋ NNA group was remarkably lower than that in HF group （ P 〈 0.01 ）. The expression of NT in hepatic tissue was the strongest and the degree of hepatic fibrosis was the most serious in HF group. CONCLUSION： ONOO^- promotes the development of hepatic fibrosis.