促红细胞生成素对失血性休克大鼠肾损伤的保护作用

Protective Effect of Erythropoietin on Renal Ischemia or Reperfusion Injury Following Hemorrhagic Shock in Rats

【目的】探讨促红细胞生成素(Erythropoietin,Epo)对失血性休克大鼠肾损伤的保护作用。【方法】建立失血性休克大鼠肾损伤模型,选用SD大鼠36只随机分为假休克组、休克组、Epo治疗组,进行组织学观察,并检测血浆丙二醛(MDA)、肌酐(Cr)、尿素氮(BUN)和肾组织匀浆超氧化物歧化酶(SOD)、白介素-6(IL-6)的变化。【结果】Epo治疗组血浆MDA、Cr、BUN水平较休克组显著下降(P<0.05);肾组织匀浆SOD显著升高I、L-6显著降低(P<0.05)。【结论】Epo对失血性休克大鼠肾损伤具有保护作用,这种作用可能与提高肾组织中SOD水平,降低肾组织中IL-6水平有关。

[Objective]To study the protective effect of erythropoietin（EPO） on renal ischemia /reperfusion（I/R） injury following hemorrhagic shock in rats. [Methods]Using hemorrhagic shock model,36 SD rats were divided into sham shocked group, shocked group and Epo group. These rats underwent the renal I/R injury developed by hemorrhagic shock for 1 hour and then reperfusion for 24 hours. Epo was used on the rat hemorrhagic shock models at the beginning of reperfusion. The changes of structure of rats were determined. Plasma MDA,Cr,BUN and tissue SOD,IL-6 were observed. [Results]Plasma MDA,Cr,BUN and tissue IL-6 of Epo groups were significantly lower than those of shocked groups. SOD in renal tissue was significantly higher and IL-6 significantly lower in EPO group（ P 〈0.05）. [Conelusion]Our results indicate that Epo has a protective effect on renal isehemia/reperfusion injury following hemorrhagic shock in rats because it can increase SOD and decrease IL-6 in renal tissue .