“双固一通”针法对绝经后骨质疏松症模型大鼠骨矿物质含量、骨密度和雌二醇的影响

Influences of “Double-reinforcing and One-unblocking” Acupuncture on the Mineral Content and the Density of Bone and Estradiol in A Rat Model of Postmenopausal Osteoporosis

目的探讨“双固一通”针法对绝经后骨质疏松症的防治作用。方法60只610月龄雌性SD大鼠,切除双侧卵巢后饲养90d,造成实验性骨质疏松症动物模型,随机分成对照组、常规取穴组、双固组、一通组、双固一通组5组。对照组不作任何治疗;常规取穴组以取脾俞、胃俞、肾俞,气海俞;双固组取关元、足三里;一通组取肾俞、膈俞、大杼;双固一通组取关元、足三里、肾俞、膈俞、大杼。电针连续治疗8w,麻醉后俯卧置于双能X线骨密度诊断仪上,检测L1-5及股骨的骨矿物质含量(BMC)和骨密度(BMD)。结果与对照组比较,所有治疗组的BMC、BMD及E2均增高(P<0.05),双固一通组显著增高(P<0.01);双固一通组较常规取穴组、双固组、一通组显著增高(P<0.05);常规取穴组、双固组、一通组三组之间比较无明显差异(P>0.05)。结论针刺能够提高绝经后骨质疏松症模型大鼠骨矿物质含量、骨密度及雌二醇水平,且“双固一通”针法优于常规方法。

Objective To investigate the preventing and treating effects of “double-reinforcing and one-unblocking” acupuncture on postmenopausal osteopomsis. Methods Sixty female SD rats aged 6-10 months were ovariectomized bilaterally and fed for 90 days to make an animal model of osteopomsis. They were randomly divided into five groups ： control, routine points selection, double-reinforcing, one-unblocking, and double-reinforcing and one-unblocking groups. The control group was not treated. Pishu （BL 20 ）, Weishu （ BL 21 ）, Shenshu （ BL 23 ） and Qihaishu （ BL 24） were selected in the routine point selection group, Guanyuan （ CV 4） and Zusanli （ST 36 ） in the double-reinforcing group, Shenshu, Geshu （ BL 27 ） and Dazhu （ BL 11 ） in the one-uublocking group and Guanyuan, Zusanli, Shenshu, Geshu and Dazhu in the double-reinforcing and one-unblocking group. Electroacupuncture was given for consecutive 8 weeks. After anesthesia, the rat was prone put on a double-energy X-ray bone densitometer and bone mineral content （BMC） and bone density （BD） were examined in L1.5 and the femur. Results As compared with the control group, BMC, BD and E2 significantly increased in all the other groups （P 〈0.05 ） and very significantly increased in the double-reinforcing and one-uublocking group （P 〈 0.01 ）. They were significantly higher in the double-reinforcing and one-unblocking group than in the routine point selection, double-reinforcing and one-unblocking groups （P 〈 0.05 ）. There was no significant difference among the routine point selection, double-reinforcing and one-unblocking groups （ P 〉 0.05 ）. Conclusion Acupuncture can raise bone mineral content, bone density and estradiol level in a rat model of postmenopausal osteoporosis and “double-reinforcing and one-uublocking” acupuncture is better than routine acupuncture.