氨基胍对大鼠急性肺栓塞后肺损伤作用的实验研究

Effect of aminoguanidine on the lung injury after acute pulmonary embolism in rats

目的观察氨基胍对大鼠急性肺栓塞后肺损伤的作用。方法采用改良的导管方法将体外已凝的大鼠血栓注入右心房制备大鼠急性肺栓塞模型。将大鼠随机分为血清对照组(Con)、肺栓塞模型组(PE)、氨基胍治疗组(AG),氨基胍治疗组于制成肺栓塞后立即腹腔注射氨基胍,模型组给等容量的生理盐水,血清对照组只通过导管注入血清。于肺栓塞后1h,放血处死大鼠,迅速取肺,测定肺系数,肺组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)的活性,血浆中NO、内皮素-1(ET-1)、血栓烷素-2(TXB2)的含量,并对肺组织常规HE染色。结果肺栓塞后,肺系数、NO、MDA、ET-1、TXB2含量明显高于血清对照组,SOD的活性明显低于血清对照组,肺动脉血管内可见纤维性栓子,小动脉扩张充血明显,肺泡萎陷,肺间质水肿并可见多量红细胞漏出,给予氨基胍治疗后,肺系数、NO、MDA、ET-1、TXB2含量明显下降,SOD的活性明显上升,且能改善病理变化。结论氨基胍对急性肺栓塞大鼠有保护作用。

Objective To investigate the effect of aminoguanldine （AG） on the lung injury after the acute pulmonary embolism in rats. Methods An experimental pulmonary embolism model in rats was made by injecting coagulated thrombus in vitro into right atrium eordis through a conductor.The rats were randomly divided into serum control group （control group）,pulmonary embolism model group （PE group） and AG group. There were six rats in each group. In AG group, AG was administrated intraperitoneally immediately after pulmonary embolism. Normal saline was injected into the rats in PE group. In control group, serum was only injected through a conductor. The rats were killed 1 h after pulmonary embolism. The lung was removed immediately. Firstly, the pulmonary coefficient was calculated （%）. Secondly, the MDA content and SOD activity in lung tissue were measured with colorimetric method. The NO content in plasma was also detected with colorimetrlc method. Endothelins-1 and thrombexane-2 in plasma were tested with radiolmmuity method. Thirdly, the lung sections were stained by HE and the changes of lung were investigated. Results The lung coefficient, NO content, MDA content, EF-1 and TXB2 levels in PE group were markedly higher than those in control group. The SOD activity was obviously lower than that in control group. Administration of AG could decrease the lung coeffficient, NO content, MDA content, ET-1 and TXB2. levels, and could increase SOD activity. In PE group fiber thrombus could be seen in the blood vessel of pulmonary artery by microscope. The small Arteries expanded, hyperemia was obvious and alveoli collapsed. The lung interstitial edema could be seen with the leaking of erythrocyte. AG could relieve pulmonary injury in rats. Conclusion AG has a definite therapeutic effect on lung injury after acute pulmonary embolism in rats.