三羧氨基喹啉对人舌鳞状细胞癌Tca8113裸鼠移植瘤效应及其机制研究

Study on the antitumor effects of Linomide in the transplant lump to the naked rat of the cancer cells Tca8113 and its related mechanisms

目的:观察三羧氨基喹啉(Linomide)对人口腔癌裸鼠移植瘤的治疗作用,并探讨其作用机制。方法:建立人舌癌裸鼠移植瘤模型,观察Linomide对肿瘤生长的抑制作用,采用免疫组化染色检测Linomide治疗组和对照组荷瘤裸鼠的肿瘤微血管密度(MVD)的变化,用半定量RT-PCR方法研究治疗组和对照组肿瘤组织中VEGF和VEGF受体FltlmRNA表达的改变,并测定各组小鼠血清VEGF水平的变化,同时,用原位末端标记(TUNEL)法观察治疗组和对照组裸鼠瘤体内肿瘤细胞凋亡的发生情况。结果:Linomide治疗组的裸鼠皮下肿瘤生长明显受到抑制,平均瘤重低于对照组(P<0.01)。结论:Linomide可有效抑制人舌鳞状细胞癌裸鼠移植瘤的生长,降低肿瘤微血管密度,通过抑制血管形成而发挥抗肿瘤作用,Linomide可抑制瘤组织VEGF受体FltlmRNA的表达;Linomide治疗荷瘤裸鼠后可诱导肿瘤细胞发生凋亡。

Objective To observe Linomide to the treatment function of the transplant lump of the naked rat of the mouth cavity cancer of person,and inquiry into its function mechanism. Method Person＇s tongue cancer transplant lump model of the naked rat was built , function of the growing tumor of Linomide to the repressment observed , lotus lumps of the tumor capillaries the variety of the density （MVD） of the naked rat of the Linomide treatment set and the matched control examined by the immunohistochemistry staining, the VEGF and VEGF Fltl mR.NA expression of change in tumor organization of treatment set and the matched control by the half metered RT-PCR , and VEGF serum level in each small rat measured ,at the same time ,we observed the occurrence circumstance the tumor cell of in the naked rat lump body in matched control and treatment set by tunel. Results The tumor growth was obviously repressed,the average lumps was heavy lower than matched control （P〈0.01） Conclusion The Linomide can repress the naked rat of the tongue scale form cell cancer of person to transplant the growth of the lump,reduce the tumor capillaries density effectively,repress the blood vessel formation to act as the exe＇rtive anti-tumor functions,the Linomide can repress the expression of VEGF Fld mRNA in lump organization ,induce the tumor cell to occurr apoptosis.