儿童肿瘤患者的染色体不稳定性研究

Chromosomal Instability in Children with Malignancies

[目的]通过对儿童染色体的稳定性研究,探讨儿童恶性肿瘤的发病机制。[方法]用姊妹染色单体互换(SCE)技术检测23例恶性肿瘤患儿的染色体稳定性。[结果]患儿外周血SCE率(10.40±2.780)明显高于正常对照组(4.47±0.771),经统计学分析有极显著性差异(P<0.001)。[结论]恶性肿瘤患儿的染色体不稳定性增加,表明DNA受损,修复能力减低,为基因突变和癌基因激活引起细胞癌变提供了条件。

[Purpose] To investigate chromosomal stability in children with malignancy and its carcinogenesis. [Methods] Chromosomal stability were detected in 23 children with malignancy by the technique of sister chromatid exchange（SCE）. [Results] The frequency of SCE in 23 children with malignancy （10.40±2.780） was higher than that in the normal controls（4.47±0.771 ） with significant difference （P〈0.001）. [Conclusion] The high frequency of sister chromatid exchange （SCE） in children with malignancy indicates the damage and reduced repairable ability of DNA. These provides cellular conditions for gene mutation and cancerous change.