大鼠肺孢子虫肺炎动物模型建立及病原学病理学观察

ESTABLISHMENT OF RAT MODEL OF PNEUMO CYSTIS PNEUMONIA AND PATHOGENIC AND PATHOLOGICAL OBSERVATIONS

纯系Wistar大鼠皮下注射醋酸可的松25mg/次,每周2次,用药7周即可成功诱发大鼠肺孢子虫肺炎。相差显微镜直接镜检、姬姆萨氏染色(Giemsa's stain)、六亚甲基四胺银染色(GMS stain)和亚甲胺蓝染色(TBO stain)均可检出包囊。其中六亚甲基四胺银染色可显示囊壁特征性的括弧样结构,具有确诊价值。病理改变主要为肺泡间隔增厚和淋巴细胞、单核细胞浸润,肺泡中少量蜂窝状渗出物。对肺孢子虫的滋养体。

Seventeen wistar rats were injected with cortisone acetate twice a week subcuteneously. After 7 weeks, pneumocystis pneumonia of rats was induced successfully. The cysts of Pneumocyst carinii were identified by phase-contrast microscope, Giemsa's stain, Gomori's methenamine silver nitrate stain and Chalvardjian's toluidine blue-O stain simultaneously. A characteristic parentheses-like structure was found by Gomori's stain in cyst wall. This structure has important value for differential diagnosis of pneumocystis pneumonia. The pathological changes were thickening of alveolar wall and infiltration of lymphocytes and monocytes. A few of honeycomb-like infiltration were found in the alveolar cavity. The Ultrastructures of trophozoite, precyst and cyst were observed by transmission electron microscope