光化学法对血液中病毒的灭活效果研究

Study on inactivation of virus in blood by photochemical methods

目的:筛选并验证全血中脂质包膜病毒灭活的最佳光化学方法。方法:以f2噬菌体为试验病毒,通过噬斑计数法筛选最佳灭活血液中病毒的光化学法,并通过正交试验筛选灭活血液中病毒的最佳作用条件;以HIV-1和日本脑炎病毒为试验病毒,通过细胞感染试验验证最佳光化学法对血液中脂质包膜病毒的灭活作用。结果:与核黄素和金丝桃素相比,亚甲基蓝(MB)所致的光化学效应对血液中f2噬菌体的灭活作用显著(P<0.05);在使血液中f2噬菌体下降超过5个对数级时,MB光化学的最佳作用条件分别是:MB终浓度为15μmol/L,可见光(640nm)照射强度为40000Lux,光照射作用时间40min;在相同MB加入剂量和光照射强度下,照射10min,血液中HIV全部灭活(下降5.78个对数级),照射20min,血液中日本脑炎病毒全部灭活(下降7.00个对数级)。结论:亚甲基蓝光化学法可高效灭活全血中f2噬菌体、HIV-1与日本脑炎病毒。

Objective： To screen and verify an optimal photochemical method to inactivate the lipid enveloped virus in blood. Methods： The bacteriophage f2 was used as testing virus, the optimal photochemical method of viral inactivation was screened by plaque count techniques and the optimal condition of viral inactivation was selected by means of orthogonal experiments. Under the optimal condition, the efficacy of inactivation of HIV-1 and Japanese encephalitis virus in blood by methylene blue（MB） photochemical method were verified by the cells infected test. Results： The efficiency of inactivation of bacteriophage f2 in blood by MB photochemical treatment was significantly higher than those by hypericin and riboflavin（p〈0.05）. If the level of phage f2 was going to be reduced over 5 Log grades, 10μmol/L MB, 40 000 Lux（640 nm） light and 40 min irradiation time were the optimal treatment condition. At the same dose of MB and intensity of irradiatioa, the levels of HIV - 1 decreased more than 5.78 Log by irradiation for 10 min and Japanese encephalitis virus decreased more than 7.00 Log by irradiation for 20 min. Conclusion： MB photochemical method can inactivate bacteriophage f2, HIV - 1 and Japanese encephalitis virus in blood effectively.