氟暴露工人血浆抗热休克蛋白70抗体表达水平的研究

Levels of Plasma Antibodies to Heat Shock Protein 70 in the Workers Exposed to the Fluorides

目的:检测氟暴露工人血浆抗热休克蛋白70(HSP70)抗体表达情况,探讨其与氟暴露应激反应的关系。方法:选择某铝厂氟暴露工人和非氟暴露工人各22人,用氟离子选择电极法测定血清氟、尿氟离子浓度,用Western-ELISA方法检测血浆抗HSP70抗体阳性率。结果:氟暴露组工人血清氟、尿氟离子浓度明显高于非暴露组(P<0.01);血浆HSP70抗体阳性率比较,氟暴露组抗HSP70抗体阳性率明显高于非暴露组(P<0.01)。结论:氟暴露环境下工人抗HSP70抗体阳性率明显增加,可能与氟暴露环境的应激保护反应有关。

Objective： To examine the levels of plasma antibodies to heat shock protein 70（HSP70） and evaluate their role in the environmental progression of the workers exposed to the fluorides. Methods： The concentrations of fluoride （Fluorine （—）） in plasma and urine samples were analysed by the ion selective electrode, Western-ELISA method was used to examine the plasma levels of anti-HSP70 antibodies in 22 exposed workers and 22 unexposed workers to the fluorides. Results： Compared to the 22 workers unexposed to the fluorides, the concentrations of fluorine-in plasma and urine samples were significantly higher,and the levels of anti-HSP70 antibodies were a significantly increased in 22 exposed workers, Conclusion： The increase of anti-HSP70 antibodies may be involved the protection of stress respone in humans exposed to the fluorides.