多角体病毒琼脂免疫双扩散和ELISA检测研究

Study of a Gel Double Immunodiffusion and I-ELISA for Detection of Spodoptera litura nucleopolyhedrosis Virus and Autographa californica Nuclear Polyhedrosis Virus

用提纯的SpltNPV、AcNPV病毒粒子分别为免疫抗原获得抗血清,以此抗血清为抗体,提纯的SpltNPV、AcNPV多角体裂解所得粗提纯病毒粒子为检测抗原,初步建立两种病毒的琼脂免疫双扩散法和ELISA间接检测方法。琼脂免疫双扩散结果表明:所得抗血清能识别粗提抗原,但不能与细胞培养的病毒粒子反应。两种病毒I-ELISA检测灵敏度分别达11.23、11.67 ng。

SpltNPV antisera and AcNPV antisera were produced by using purified SpltNPV and AcNPV virion immuned rabbit. Double immunodiffusion and an I-ELISA were established by using antisera as antibody and crude purified virion as detect antigen. The results of double immunodiffusion indicated that cross reactions were detected between antisera and crude purified virion, and not between antisera and cell cultured virion. The sensitivity of the immunoassay of two viruses was 11. 23 ng and 11.67 ng.