参麦注射液预防大鼠化学性肝硬化作用及机制

Preventive effect and mechanism of Shenmai injection on hepatocirrhosis induced by chemical damage in rat

目的研究参麦注射液对四氯化碳(carbontetrachloride,CTC)实验性肝硬化的预防作用及可能机制。方法将成年健康SD大鼠随机分成空白对照组、CTC组、参麦注射液(Shenmaiinjection,SMI)组。对照组皮下注射花生油3mL/kg,后两组皮下注射50%CTC油3ml/kg,每周两次,共8周,SMI保护组每天给予参麦注射液1mL/kg皮下注射,于第4周、第8周,每组各处死8只大鼠,取肝组织测定脂质过氧化物代谢产物丙二醛(malondialdehyde,MDA)、一氧化氮(nitricoxide,NO)及羟脯氨酸(Hydroxyproline,Hyp);取下腔静脉血测定血清丙氨酸氨基转移酶(ALT)、天门冬酸氨基转移酶(AST)和测大鼠门静脉压力(portalveinpres-sure,PVP);并观察肝脏显微结构的变化。结果与CTC组相比,SMI组肝组织Hyp、MDA含量明显低(P<0.01);NO含量明显高(P<0.01);血清ALT、AST含量及大鼠PVP明显低(P<0.01),且肝细胞损害、肝脂肪变性、炎症细胞浸润及纤维组织增生均较轻。结论SMI对CTC所致的肝脏损伤有明显的保护作用,能明显抑制肝细胞中胶原纤维增生,降低PVP,预防肝硬化。

[Objective] To study the preventive effect and mechanism of Shenmai injection （SMI） on hepatocirrhosis induced by carbon tetrachloride（CTC）. [Methods] Sixty SD rats were randomly divided into normal control group （n=16）, CTC group （n=22）, SMI protection group （n=22）. Subcutaneous injection of oil 3 mL/kg was given twice a week to normal control group rats, Subcutaneous injection of 50% CTC oil 3 mL/kg was given twice a week to the latter two group rats, 1ml/kg SMI was given to SMI protection group by subcutaneous injection every day, the eight rats of each group were executed at the fourth week, the eighth week.Hydroxyproline（Hyp）, nitric oxide （NO） and malondialdehyde （MDA） in liver tissue and the ALT, AST levels in the blood and portal vein pressure （PVP） were examined and the microstructure of the liver was observed. [Results] Compared with CTC group, the serum AST and ALT, the liver tissue Hyp and MDA, and PVP in rats significantly decreasing （P 〈 0.01）; the liver contents of NO significantly increased （P 〈0.01） in SMI protection group ,and the extent of liver damages, fatty degeration, infiltration of inflammatory cells and the formation of fibroblasts and collagenous fibers were less severe in SMI protection group. [Conclusion] SMI has a significant preventive effect on the rat liver of chemical damage and on the rat hepatocirrhosis by inhibiting the formation of fibroblasts and collagenous fibers in liver tissues and reducing PVP.