醇浓度对醇法大豆浓缩蛋白浸提液中溶出蛋白的影响

The Effects of Alcohol Content on the Extraction of ALSPC

利用不同浓度乙醇对低变性脱脂大豆粕进行醇法浸提,制取大豆浓缩蛋白。对浸提液采用SDS-PAGE电泳法测定蛋白质分子量,并对蛋白质溶出率进行测定,探索不同醇浓度对浸提液中蛋白溶出率及分子量的影响,为醇法制取大豆浓缩蛋白浸提液再加工、回收蛋白提供了一定的理论基础。

The effect of different alcohol content in protein extraction on the molecular weight of soybean protein concentrate was systematically studied by using SDS - PAGE electrophoresis and by examining protein dissolution rate. This study results in appropriate processing condition and provides theoretical basis for development of protein reprocessing.