鲟鱼软骨对小鼠生长及肝损伤的保护作用研究

Effects of Sturgeon Cartilage on Growth and Its Protective Effect on CCl_4-induced Liver Injury of Mice

鲟鱼软骨按0%、1%和3%的添加比例制成3种试验饲料.用健康的雄性昆明小白鼠为实验对象,分别进行4周的生长试验和CC l4染毒试验.CC l4染毒试验于28 d时,对照组腹腔注射精制豆油,其余各组以10 mL.kg-1剂量注射0.2%CC l4精制豆油溶液造成肝损伤,36 h后眼眶取血测定转氨酶活性,并取肝、脑、睾丸,测定超氧化歧化酶(SOD)活性、Na+,K+-ATP酶活性、总抗氧化值(T-AOC)、丙二醛(MDA).小鼠生长试验结果表明,软骨组小鼠体增重增加、耗料增重比降低及组织系数变化均与对照组没有明显差异(P>0.05).但软骨组小鼠血浆AST活性显著升高(P<0.05).小鼠CC l4染毒试验结果表明,与CC l4肝损伤组比较,软骨组小鼠肝、脑和睾丸组织中的Na+,K+-ATP酶活性、SOD活性、T-AOC值呈不同程度的升高,而MDA含量则呈不同程度的降低,但差异不明显(P>0.05).提示,鲟鱼软骨能够对小鼠的生长代谢产生影响并对CC l4所致小鼠肝损伤有一定的保护作用.

Two experiments were conducted. Three testing diets with 0 %, 1% and 3 % of sturgeon Acipenser schrenckii cartilage addition were used to determine the effect on the growth performance in the first experiment and the response of antioxidation to CC14-induced liver injury of mice in the second experiment. The trials were lasted for four weeks. In the second trial, 0. 2 % CC14 10 ml/kg BW was given to induce liver injury at the 28th day of successive feeding. After 36 h, blood was collected for transaminases analysis, then, liver, brain or testis tissues were caught to determine superoxide dismutase （SOD） activity, total antioxidative competency （T-AOC）, malondialdehyde （MDA） content and Na^＋ ,K^＋-ATPase activity. The resuits showed that there were no differences among groups in both growth performance and tissue coefficients, but plasma AST activity in treated groups were significantly higher than that in control. Compared with control with CC14-induced liver injury of mice, Na^＋ ,K^＋-ATPase, SOD activity, and T-AOC of the treated groups generally increased slightly while MDA content decreased a little. It can be inferred that sturgeon cartilage can affect some metabolism and protect against CCl4-induced hepatotoxicity.